socs3  (Servicebio Inc)

Journal: PLOS Pathogens

Article Title: Glutamine alleviates Staphylococcus aureus -induced mastitis by modulating macrophage polarisation

doi: 10.1371/journal.ppat.1014053

Figure Lengend Snippet: RAW264.7 cells were cultured in αKG-supplemented medium, treated with the STAT3 inhibitor Stattic, and subsequently stimulated with S. aureus . A: Protein expression levels of the JAK1/STAT3 signaling pathway and its negative regulator SOCS3 were detected in the Control, αKG, and αKG + S. au groups. B-I: Relative mRNA expression of M2 and M1 marker genes across the four experimental groups. J-L: Inflammatory cytokine levels and NF-κB signaling pathway activity in the Control, αKG, αKG + S. au , and αKG + S. au +Stattic groups. M: Expression levels of the JAK1/STAT3 signaling pathway across the four experimental groups. The data are presented as mean ± SD. One-way analysis of variance was performed for statistical analysis. * P < 0.05, ** P < 0.01 and *** P < 0.001 indicate significance from each group. ns, no significance.

Article Snippet: The specific primary antibodies used in the experiment included p65 (#8242, Cell Signaling Technology, USA), p-p65 (#3033, Cell Signaling Technology, America), IκB (AF5002, Affinity Biosciences, Jiangsu, China), p-IκB (AF2002, Affinity Biosciences, Jiangsu, China), ZO-1 (AF5145, Affinity Biosciences, Jiangsu, China), Occludin (DF7504, Affinity Biosciences, Jiangsu, China), Claudin-3 (AF0129, Affinity Biosciences, Jiangsu, China), ATG5 (bsm-52596R, Bioss, Woburn, MA, USA), LC3-I/LC3-II (AF5402, Affinity Biosciences, Jiangsu, China), p62 (ab91526, Abcam plc, Cam bridge, UK), SOCS3 (bs-0580R, Bioss, Woburn, MA, USA), JAK1 (bs-1439R , Bioss, Woburn, MA, USA), p-JAK1 (bs-3238R, Bioss, Woburn, MA, USA), STAT3 (AF6294, Affinity Biosciences, Jiangsu, China), p-STAT3 (AF3293, Affinity Biosciences, Jiangsu, China) and β-actin (T0022, Affinity Biosciences, Jiangsu, China).

Techniques: Cell Culture, Expressing, Control, Marker, Activity Assay

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Application of 18 β -glycyrrhetinic acid Fluorescent probes in cell imaging

doi: 10.1080/14756366.2026.2631869

Figure Lengend Snippet: Effects of 18 β -GA-PAL-ABP on LPS-induced release of HDAC8, STAT3, P-STAT3, and SOCS3. A: expression of HDAC8 protein; B: expression of STAT3 protein; C: expression of P-STAT3 protein; and D: expression of SOCS3 protein. E: The protein expression map in RAW 264.7 cells treated with Class I compounds and drug concentrations; F: The protein expression map in RAW 264.7 cells treated with Class II compounds and drug concentrations; G: The protein expression map in RAW 264.7 cells treated with Class III compounds and drug concentrations. Compared to the Control group, # p < 0.05, ## p < 0.01, ### p < 0.001. Compared to the Model group, * p < 0.05, ** p < 0.01, *** p < 0.001. Compared to the 18β-GA group, + p < 0.05, ++ p < 0.01, +++ p < 0.001.

Article Snippet: GAPDH (8884, CST, 1:5000), HDAC8 (#66042, CST, 1:1500), P-STAT3 (#9154, CST, 1:1500), STAT3 (#12640, CST, 1:1000), SOCS3 (#52113, CST, 1:2000), SDS-PAGE reagents (M00657, Genescript, Nanjing, China).

Techniques: Expressing, Control